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pcdna3 her2 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 her2 plasmid
    Pcdna3 Her2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+her2+plasmid/pcDNA3-HER2-DMVF-181-N+(Plasmid+%2387622)/pmc11337831-416-6-8
    Average 90 stars, based on 5 article reviews
    pcdna3 her2 plasmid - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody–drug conjugate–resistant HER2-overexpressing breast cancer
    Article Snippet: .. SUM190-TDXdR and HCC1954-TDXdR cells transfected with pcDNA3-HER2 plasmid (Addgene, Watertown, MA, USA) using the Neon transfection kit (ThermoFisher) and underwent an SRB proliferation assay with T-DXd. ..

    Article Title: Therapeutic efficacy of SYM004, a mixture of two anti-EGFR antibodies in human colorectal cancer with acquired resistance to cetuximab and MET activation
    Article Snippet: For immunoprecipitation 2 mg of protein lysates were immune-precipitated with the required antibodies; immune-complexes were recovered with protein G Sepharose (Roche Diagnostics) and detected by Western blotting. .. SW48 cells were transfected with 5μg of pcDNA3 HER2 plasmid (#16257, Addgene, Cambridge, MA, USA) using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA) following manufacturer's instructions. ..

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody-drug conjugate-resistant HER2-overexpressing breast cancer.
    Article Snippet: .. SUM190‐TDXdR and HCC1954‐TDXdR cells transfected with pcDNA3‐HER2 plasmid (Addgene, Watertown, MA, USA) using the Neon transfection kit (ThermoFisher) and underwent an SRB proliferation assay with T‐DXd. ..

    Plasmid Preparation:

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody–drug conjugate–resistant HER2-overexpressing breast cancer
    Article Snippet: .. SUM190-TDXdR and HCC1954-TDXdR cells transfected with pcDNA3-HER2 plasmid (Addgene, Watertown, MA, USA) using the Neon transfection kit (ThermoFisher) and underwent an SRB proliferation assay with T-DXd. ..

    Article Title: Functional cooperation between co-amplified genes promotes aggressive phenotypes of HER2-positive breast cancer
    Article Snippet: .. To generate a lentiviral construct expressing HER2, the target segment was amplified by PCR using pcDNA3-HER2 plasmid (Addgene plasmid #16257) as the template. .. The PCR product was digested with restriction enzymes Nhe I and Not I, cloned into lentiviral vector pCDH-CMV (System Biosciences) and the sequence was confirmed by sequencing (Genewiz).

    Article Title: Therapeutic efficacy of SYM004, a mixture of two anti-EGFR antibodies in human colorectal cancer with acquired resistance to cetuximab and MET activation
    Article Snippet: For immunoprecipitation 2 mg of protein lysates were immune-precipitated with the required antibodies; immune-complexes were recovered with protein G Sepharose (Roche Diagnostics) and detected by Western blotting. .. SW48 cells were transfected with 5μg of pcDNA3 HER2 plasmid (#16257, Addgene, Cambridge, MA, USA) using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA) following manufacturer's instructions. ..

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody-drug conjugate-resistant HER2-overexpressing breast cancer.
    Article Snippet: .. SUM190‐TDXdR and HCC1954‐TDXdR cells transfected with pcDNA3‐HER2 plasmid (Addgene, Watertown, MA, USA) using the Neon transfection kit (ThermoFisher) and underwent an SRB proliferation assay with T‐DXd. ..

    Sulforhodamine B Assay:

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody–drug conjugate–resistant HER2-overexpressing breast cancer
    Article Snippet: .. SUM190-TDXdR and HCC1954-TDXdR cells transfected with pcDNA3-HER2 plasmid (Addgene, Watertown, MA, USA) using the Neon transfection kit (ThermoFisher) and underwent an SRB proliferation assay with T-DXd. ..

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody-drug conjugate-resistant HER2-overexpressing breast cancer.
    Article Snippet: .. SUM190‐TDXdR and HCC1954‐TDXdR cells transfected with pcDNA3‐HER2 plasmid (Addgene, Watertown, MA, USA) using the Neon transfection kit (ThermoFisher) and underwent an SRB proliferation assay with T‐DXd. ..

    Proliferation Assay:

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody–drug conjugate–resistant HER2-overexpressing breast cancer
    Article Snippet: .. SUM190-TDXdR and HCC1954-TDXdR cells transfected with pcDNA3-HER2 plasmid (Addgene, Watertown, MA, USA) using the Neon transfection kit (ThermoFisher) and underwent an SRB proliferation assay with T-DXd. ..

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody-drug conjugate-resistant HER2-overexpressing breast cancer.
    Article Snippet: .. SUM190‐TDXdR and HCC1954‐TDXdR cells transfected with pcDNA3‐HER2 plasmid (Addgene, Watertown, MA, USA) using the Neon transfection kit (ThermoFisher) and underwent an SRB proliferation assay with T‐DXd. ..

    Construct:

    Article Title: Functional cooperation between co-amplified genes promotes aggressive phenotypes of HER2-positive breast cancer
    Article Snippet: .. To generate a lentiviral construct expressing HER2, the target segment was amplified by PCR using pcDNA3-HER2 plasmid (Addgene plasmid #16257) as the template. .. The PCR product was digested with restriction enzymes Nhe I and Not I, cloned into lentiviral vector pCDH-CMV (System Biosciences) and the sequence was confirmed by sequencing (Genewiz).

    Expressing:

    Article Title: Functional cooperation between co-amplified genes promotes aggressive phenotypes of HER2-positive breast cancer
    Article Snippet: .. To generate a lentiviral construct expressing HER2, the target segment was amplified by PCR using pcDNA3-HER2 plasmid (Addgene plasmid #16257) as the template. .. The PCR product was digested with restriction enzymes Nhe I and Not I, cloned into lentiviral vector pCDH-CMV (System Biosciences) and the sequence was confirmed by sequencing (Genewiz).

    Amplification:

    Article Title: Functional cooperation between co-amplified genes promotes aggressive phenotypes of HER2-positive breast cancer
    Article Snippet: .. To generate a lentiviral construct expressing HER2, the target segment was amplified by PCR using pcDNA3-HER2 plasmid (Addgene plasmid #16257) as the template. .. The PCR product was digested with restriction enzymes Nhe I and Not I, cloned into lentiviral vector pCDH-CMV (System Biosciences) and the sequence was confirmed by sequencing (Genewiz).

    Polymerase Chain Reaction:

    Article Title: Functional cooperation between co-amplified genes promotes aggressive phenotypes of HER2-positive breast cancer
    Article Snippet: .. To generate a lentiviral construct expressing HER2, the target segment was amplified by PCR using pcDNA3-HER2 plasmid (Addgene plasmid #16257) as the template. .. The PCR product was digested with restriction enzymes Nhe I and Not I, cloned into lentiviral vector pCDH-CMV (System Biosciences) and the sequence was confirmed by sequencing (Genewiz).



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